Assay: Fatty Acids (Non-esterified)
Targeted method quantifying up to 29 free fatty acids (non-esterified) using LC-MS/MS with MRM. Quantitation is performed using calibrations of up to 10 concentration levels and 6 labeled surrogate internal standards. Standard reference material (SRM) 1950 containing certified and reference values for fatty acids is also analyzed with each sample batch for QC purposes.
| Name | Alternative Name | Carbons:Double Bonds | InChIKey |
| Decanoic acid | Caproic acid | 10:0 | FUZZWVXGSFPDMH-UHFFFAOYSA-N |
| Dodecanoic acid | Lauric acid | 12:0 | POULHZVOKOAJMA-UHFFFAOYSA-N |
| Tetradecanoic acid | Myristic acid | 14:0 | TUNFSRHWOTWDNC-UHFFFAOYSA-N |
| 9-Tetradecenoic acid | Myristoleic acid | 14:1 | YWWVWXASSLXJHU-WAYWQWQTSA-N |
| 13-methyltetradecanoic acid | 15:0 | ZOCYQVNGROEVLU-UHFFFAOYSA-N | |
| Pentadecanoic acid | 15:1 | WQEPLUUGTLDZJY-UHFFFAOYSA-N | |
| Hexadecanoic acid | Palmitic acid | 16:0 | IPCSVZSSVZVIGE-UHFFFAOYSA-N |
| 9-Hexadecenoic acid | Palmitoleic acid | 16:1 | SECPZKHBENQXJG-FPLPWBNLSA-N |
| 15-methylhexadecanoic acid | 17:0 | IIUXHTGBZYEGHI-UHFFFAOYSA-N | |
| Octadecanoic acid | Stearic acid | 18:0 | QIQXTHQIDYTFRH-UHFFFAOYSA-N |
| 9-Octadecenoic acid | Oleic acid | 18:1 | ZQPPMHVWECSIRJ-KTKRTIGZSA-N |
| 9,12-Octadecadienoic acid | Linoleic acid | 18:2 | OYHQOLUKZRVURQ-HZJYTTRNSA-N |
| 9,12,15-Octadecatrienoic acid (Alpha) | Linolenic Acid | 18:3 | DTOSIQBPPRVQHS-PDBXOOCHSA-N |
| 9,12,15-Octadecatrienoic acid (Gamma) | Linolenic Acid | 18:3 | DTOSIQBPPRVQHS-PDBXOOCHSA-N |
| Nonadecanoic acid | 19:0 | YOKHLRHWEXTWJR-KHPPLWFESA-N | |
| Eicosanoic acid | Arachidic acid | 20:0 | VKOBVWXKNCXXDE-UHFFFAOYSA-N |
| 11-Eicosenoic acid | Gondoic Acid | 20:1 | BITHHVVYSMSWAG-KTKRTIGZSA-N |
| 11,14 Eicosadienoic acid | 11,14 Eicosadienoic acid | 20:2 | XSXIVVZCUAHUJO-AVQMFFATSA-N |
| 8,7,14-eicosatirenoic acid | Dihomo-Gamma-Linolenic Acid | 20:3 | HOBAELRKJCKHQD-QNEBEIHSSA-N |
| 5,8,11,14-Eicosatetraenoic acid | Arachidonic Acid | 20:4 | YZXBAPSDXZZRGB-DOFZRALJSA-N |
| 5,8,11,14,17-Eicosapentaenoic acid | EPA | 20:5 | JAZBEHYOTPTENJ-JLNKQSITSA-N |
| Docosanoic acid | Behenic Acid | 22:0 | UKMSUNONTOPOIO-UHFFFAOYSA-N |
| (13Z)-Docos-13-enoic acid | Erucic acid | 22:1 | DPUOLQHDNGRHBS-KTKRTIGZSA-N |
| 7,10,13,16-Docosatetraenoic acid | Adrenic Acid | 22:4 | TWSWSIQAPQLDBP-DOFZRALJSA-N |
| 7,10,13,16,19-docosapentaenoic acid | Clupanodonic acid | 22:5 | YUFFSWGQGVEMMI-JLNKQSITSA-N |
| 4,7,10,13,16,19-Docosahexaenoic acid | DHA | 22:6 | MBMBGCFOFBJSGT-KUBAVDMBSA-N |
| Tricosanoic acid | Tricosylic acid | 23:0 | XEZVDURJDFGERA-UHFFFAOYSA-N |
| Tetracosanoic acid | Tetracosanoic acid | 24:0 | QZZGJDVWLFXDLK-UHFFFAOYSA-N |
| 15-Tetracosenoic acid | Nervonic Acid | 24:1 | GWHCXVQVJPWHRF-KTKRTIGZSA-N |