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Roy J. Carver Biotechnology Center

DNA

HTDNA Group

MiSeq Flowcell

At Computer

Magnetic Plate

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IllinoisThe primary mission of the DNA Services Lab is to provide outstanding service and state-of-the-art instrumentation to support basic, environmental, agricultural, and translational medical research for our campus investigators and external academic and corporate collaborators.

 

Instruments


Applications

Instrumentation

NovaSeq 6000

Illumina NovaSeq 6000

This is the latest instrument from Illumina for ultra-high throughput sequencing of genomic DNA, RNA-Seq, bisulfite-treated DNA, targeted and mate-pair libraries and any sample that needs high depth or projects that involve a large number of samples.

  • S2 flowcell (2 lanes) =
    • Single- or paired-reads available on 100-cycle kit
    • Sequenced as 2x50bp paired- or 100bp single-read
    • 1.6 to 2 billion single-reads per lane
    • 3.3 to 4.1 billion paired-reads per lane
    • Up to 200Gb per lane
  • S4 flowcell (4 lanes) =
    • Paired-reads, 150bp in length on 300-cycle kit
    • 4 to 5 billion paired-reads per lane
    • Up to 750Gb per lane
 
Equipment

Illumina HiSeq 4000

For sequencing of all types of libraries: RNA-Seq, shotgun genomic, mate-pairs, ChIP-Seq, small RNA, bisulfite-treated, custom libraries, etc.

  • Single- or paired-reads, 50bp to 150bp in length
  • Each lane produces:
    • 300 to 400 million single-reads
    • 650 to 800 million paired-reads
    • Up to 120Gb per lane
  • Flowcells have 8 lanes

 
Equipment

Illumina HiSeq 2500

For sequencing of all types of libraries: RNA-Seq, shotgun genomic, mate-pairs, ChIP-Seq, small RNA, custom libraries, etc.

  • Single- or paired-reads, 50bp to 250bp in length (longest of all Illumina's high-throughput instruments).
  • Each lane produces:
    • 150 to 200 million single-reads
    • 280 to 400 million paired-reads
    • Up to 75Gb per lane
  • Flowcells have just 2 lanes and can be utilized for rapid turn-around time.
 
MiSeq

Illlumina MiSeq

 For sequencing libraries from amplicons (ie: 16S, 18S, ITS, etc), genomic DNA, small RNAs, RNA-Seq, ChIP-Seq, etc.

  • Single- or paired-reads, 50bp to 300bp in length
  • V2 Nano run produces 500k to 2 million paired-reads
  • V2 bulk run produces 8 to 20 million paired-reads
  • V3 run produces 10 to 40 million paired-reads
  • Flowcell is just 1 lane
 
GridION

Oxford Nanopore GridION

Long sequencing reads from genomic DNA, RNA, cDNA, 16S or other amplicons.

  • One flowcell produces from 200k to 3 million reads depending on type of sample
  • Reads are typically from 5kb up to 30kb, longer reads are also possible
  • Each flowcell runs one sample or pool
 
10x

10x Genomics

For both DNA and single-cell RNA-Seq. The 10x Chromium system produces libraries for de novo genome assembly, genome phasing, characterization of large structural variation and for 3' transcriptome libraries from single cells.

  • Libraries constructed on 10x are fully compatible with Illumina sequencing.
  • Fast and low cost de novo assembly of genomes.
  • Phasing of genomes and characterization of small and large structural variants.
  • de novo libraries are sequenced 2x150nt on HiSeq and typically require just 1 lane of sequencing.
  • Single-cell libraries can be constructed from 500 to 10,000 cells per library.
  • Single-cell libraries are sequenced 2x100nt or 2x150nt on the HiSeq, with 100k reads per cell recommended.