Roy J. CarverUniversity of Illinois
Biotechnology Center

TRANSGENIC MOUSE PRODUCTION

Transgenic mice are produced by injecting the DNA of interest into one-cell mouse embryos followed by implantation into foster mice. The facility will purify the transgene insert from a plasmid vector and microinject the purified DNA into mouse embryos. The gestation period of a mouse is approximately 20 days. Pups will be transferred to the investigator for genotyping at 3 weeks of age.

Time line for transgenic mouse production: 

Day 1: DNA injection

Day 21: Pups Born

Day 42: Wean & transfer pups → Breeding

To request service from Transgenic Mouse Facility:

Provide the facility with DNA construct approval date from the Institutional Biosafety Committee (IBC). The facility will not initiate any experiment without the relevant approval date.

Obtain approved protocol for animal research from IACUC. The facility will not initiate any experiment without an authorized animal research protocol number.

Arrange an appointment with the facility Director, Fuming Pan (244-0649, fpan@illinois.edu) to discuss and initiate the project.

Complete and submit the Service Request Form for pronuclear microinjection, provide the facility with 20ug of digested plasmid DNA at 0.5ug/ul or higher with a picture of the DNA prepared for fragment isolation.

The TMF will use C57BL/6 or B6C3F1 mice as egg donors, unless specially requested by investigator

The TMF will provide at least 2 transgenic founder mice per construct.

Investigators can purify their DNA inserts. We do not guarantee any transgenic mouse production using the purified DNA directly from investigators.

All the offspring in the Transgenic Mouse Facility and all the cage cost will be transferred to the investigator’s account at the weaning day

All work performed by the Roy J. Carver Biotechnology Center (CBC) should be acknowledged in scholarly publications, posters, and presentations. Proper recognition allows us to measure the impact of our work and supports our initiatives in obtaining sponsored funding. In addition, any CBC personnel who make a substantial intellectual or experimental contribution are deserving of further recognition as co-author.