Roy J. Carver
Biotechnology Center

Fragment Analysis services

For Fragment Analysis, we accept unpurified (unless otherwise directed) PCR products generated by microsatellite, AFLP, RFLP, TRFLP, ARISA or other protocols. Our capillary instruments can analyze fluorescently-labeled fragments up to 1200bp in length, and they provide high run-to-run consistency with low background noise. For sample analysis, we provide our customers with free access to a shared copy of the GeneMapper software via remote connection to a computer at the Core Facility.  Our typical turn-around time is two business days from the date of submission, and all orders are completed on a first-come, first-served basis, regardless of affiliation.
 

Size Standards

Several size standards are available to analyze fragments with 3-dye or 4-dye multiplexing. Size standards are provided by our facility and do not need to be purchased separately. Please contact us if you would like to use a custom size standard.

Submission Information

We provide one free 96-well test plate of serial dilutions to determine optimal DNA concentrations for each new customer.  Please see the Sample Submission Guidelines on the CoreLims website for information about choosing size standards and primer dye labels, making a dilution plate, submitting orders and accessing analysis software.  Due to the limitations of our capillary electrophoresis platform, we cannot successfully perform temperature-dependent analyses such as DGGE or SSCP.

Pricing

Prices include size standard, electrophoresis on the AB 3730xl and access to GeneMapper analysis software.  To receive the medium-throughput price, samples must be submitted in 96-well plates.

• Low-throughput price:  $2.80 per sample.
• Medium-throughput price:  $88.00 per 96-well plate.

This is an abridged list of our service prices and subject to change at any time. Please contact Core staff at 217.333.9520 or dna-seq@illinois.edu for further details. If you are an off-campus customer, please complete the Non-UI user form and submit with your first order.

Facility users other than State of Illinois institutions are subject to a 28.6% indirect cost rate for federally funded projects.  For off-campus projects exceeding $5,000 a contract must be negotiated through University of Illinois Grants and Contracts.  For further information contact Dr. Mark A. Mikel (217.244.0144, mmikel@illinois.edu), Associate Director of the Roy J. Carver Biotechnology Center.